Whole-Exome Sequencing to Identify Somatic Variants in Cancer - Yardena Samuels(from NHGRI)
Next Generation Sequencing
Determine genetic map position of SNP':
online tool
Conway Institute Bioinformatics Service
An overview of NGS technology and platforms:
http://www.seqwright.com
Blog on NGS technology (www.yuzuki.org) by Dale Yuzuki:
Next Generation Technologist
How to remove duplicates reads using picard:
Next Generation Sequencing
Determine genetic map position of SNP':
online tool
Conway Institute Bioinformatics Service
An overview of NGS technology and platforms:
http://www.seqwright.com
Blog on NGS technology (www.yuzuki.org) by Dale Yuzuki:
Next Generation Technologist
How to remove duplicates reads using picard:
java -Xmx2g -jar /usr/local/picard-tools/MarkDuplicates.jar
MAX_FILE_HANDLES_FOR_READ_ENDS_MAP=1000
METRICS_FILE=out.metrics
REMOVE_DUPLICATES=true
ASSUME_SORTED=true
VALIDATION_STRINGENCY=LENIENT
INPUT=input.sorted.bam
OUTPUT=out.dedup.bam
BAM file reheader trick (that works):
java -jar /home/zhengyuan/picard-tools-1.105/AddOrReplaceReadGroups.jar \
I=test.srt.bam O=test.srt.rhd.bam LB=wzy PL=illumina PU=wzy SM=wzy
samtools index test.srt.rhd.bam
Then you can proceed for others tasks like GATK coverage analysis.
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