The NextSeq 500 has a new two-dye system for measuring base-incorporation, with red dye measuring C, green dye measuring T, a mix of dyes measuring A and no dye measuring G. I think it’s the latter two that are causing most concern. I’ve little doubt that this new system will introduce bias. Sure, it will probably be solved by chemistry and software upgrades, but when?
excerpt from https://biomickwatson.wordpress.com/2014/01/17/7-reasons-why-the-nextseq-500-is-a-strange-choice/
No comments:
Post a Comment